The Comparison of Degradation Time, Weight Loss and Surface Fibrin Structure Between Gauze-Compression and Heat-Compression Platelet-Rich Fibrin Membrane
Objective: To evaluate the effect of heat-compression to platelet-rich fibrin (PRF) membrane in degradation time, weight loss and to examine surface fibrin structure using scanning electron microscope (SEM).
Methods: Sixty PRF membranes that were prepared from human blood (ten healthy volunteers). Samples were compressed at various temperatures and were arranged into six groups: control (room temperature), 60, 70, 80, 90 and 100°C. Nine of the ten samples from each group were evaluated for their degradation time and weight loss. One remaining sample was examined the surface fibrin structure using SEM.
Results: The 90 and 100°C groups had significantly different degradation times compared to those in the control, 60 and 70°C groups (p<0.05). There was no significant difference in the mean weight percentage among the six groups on each day (p<0.05). But when compared among the days in the same group, the 90 and 100°C groups were significantly different in the early phase to 11 and 6 days, respectively. The surface fibrin structure in the 90 and 100°C groups showed the least interfibrous space and lowest porosity.
Conclusions: The 90 and 100°C groups had significantly longer degradation times and delayed the early stage of degradation. It might be applied in surgical operations that need PRF membrane stability in the early phase.
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