The Efficacy of a Microplate Spectrophotometer for the Detection of Hydrogen Peroxide from Bleaching Agents
Hydrogen peroxide from bleaching agents can easily penetrate through the tooth structure into the pulpal cavity and may cause damage to pulpal cells. The objective of this study was to evaluate the amount of hydrogen peroxide penetration through the bovine tooth disc into the artificial pulp chamber after bleaching with three different bleaching agents for 1 hour. Bovine tooth discs with a thickness of 3.2 mm were prepared and placed into a modified artificial pulp chamber filled with acetate buffer solution. The specimens were divided into 3 groups and bleached with one of following bleaching agents: group-1, 40% Opalescence™ Boost (hydrogen peroxide); group-2, 20% Opalescence™ PF (carbamide peroxide); and group-3, 10% Opalescence™ PF (carbamide peroxide). The amount of peroxide that penetrated into the artificial pulp chamber was evaluated using either a standard spectrophotometer with cuvette reader or microplate spectrophotometer after 1 hour of bleaching. An increase in the amount of hydrogen peroxide penetration corresponded to an increase in the concentration of bleaching products. The highest amount of hydrogen peroxide penetration was found in the group bleached with 40% hydrogen peroxide (0.0185 mg/ml for standard spectrophotometer, 0.1080 mg/ml for microplate spectrophotometer). The microplate spectrophotometer exhibited greater efficacy for detecting the amount of hydrogen peroxide compared with the standard spectrophotometer.
1. Fearon J. Tooth whitening: concepts and controversies. J Ir Dent Assoc 2007; 53(3): 132-140.
2. Cooper JS, Bokmeyer TJ, Bowles WH. Penetration of the pulp chamber by carbamide peroxide bleaching agents. J Endod 1992; 18(7): 315-317.
3. Guenal I, Sidoti-de Fraisse C, Gaumer S, Mignotte B. Bcl-2 and Hsp27 act at different levels to suppress programmed cell death. Oncogene 1997; 15(3): 347-360.
4. Costa CA, Riehl H, Kina JF, Sacono NT, Hebling J. Human pulp responses to in-office tooth bleaching. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109(4): 59-64.
5. Aussen BS, Kingma Boltjes TY, Wostmann BS. Some fundamental remarks on the polarographic determination of the catalase activity and the H2O2 production of bacteria. Antonie van Leeuwenhoek 1950; 16(3): 65-78.
6. Mottola HA, Simpson BE, G G. Absorptiometric determination of hydrogen peroxide in submicrogram amounts with leuco crystal violet and peroxidase as catalyst. Analyt Chem 1970; 42: 410-411.
7. Kwon SR, Wertz PW. Review of the Mechanism of Tooth Whitening. J Esthet Restor Dent 2015; 27(5): 240-257.
8. Lou EK, Damiani F, Heithersay GS. Evaluation of Hydroxyl Radical Diffusion and Acidified Thiourea as a Scavenger during Intracoronal Bleaching. J Endod 2016; 42(7): 1126-1130.
9. Gerber F, Krummen M, Potgeter H, et al. Practical aspects of fast reversed-phase high-performance liquid chromatography using 3 microm particle packed columns and monolithic columns in pharmaceutical development and production working under current good manufacturing practice. J Chromatogr A 2004; 1036(2): 127-133.
10. Gimeno P, Bousquet C, Lassu N, et al. High-performance liquid chromatography method for the determination of hydrogen peroxide present or released in teeth bleaching kits and hair cosmetic products. J Pharm Biomed Anal 2015; 107: 386-393.
11. Marson FC, Goncalves RS, Silva CO, et al. Penetration of hydrogen peroxide and degradation rate of different bleaching products. Oper Dent 2015; 40(1): 72-79.
12. Camargo SE, Valera MC, Camargo CH, Gasparoto Mancini MN, Menezes MM. Penetration of 38% hydrogen peroxide into the pulp chamber in bovine and human teeth submitted to office bleach technique. J Endod 2007; 33(9): 1074-1077.
13. Nathoo SA. The chemistry and mechanisms of extrinsic and intrinsic discoloration. J Am Dent Assoc 1997; 128Suppl: 6S-10S.
14. Joiner A. The bleaching of teeth: a review of the literature. J Dent 2006; 34(7): 412-419.
15. Haywood VB. History, safety, and effectiveness of current bleaching techniques and applications of the nightguard vital bleaching technique. Quintessence Int 1992; 23(7): 471-488.
16. Dahl JE, Pallesen U. Tooth bleaching--a critical review of the biological aspects. Crit Rev Oral Biol Med 2003; 14(4): 292-304.
17. Eimar H, Marelli B, Nazhat SN, et al. The role of enamel crystallography on tooth shade. J Dent 2011; 39Suppl3: e3-10.
18. Cavalli V, Rodrigues LK, Paes-Leme AF, et al. Effects of the addition of fluoride and calcium to low-concentrated carbamide peroxide agents on the enamel surface and subsurface. Photomed Laser Surg 2011; 29(5): 319-325.
19. Watt BE, Proudfoot AT, Vale JA. Hydrogen peroxide poisoning. Toxicol Rev 2004; 23(1): 51-57.
20. Goncalves RS, Costa CA, Soares DG, dos Santos PH, Cintra LT, Briso AL. Effect of Different Light Sources and Enamel Preconditioning on Color Change, H2O2 Penetration, and Cytotoxicity in Bleached Teeth. Oper Dent 2016; 41(1): 83-92.
21. Marson FC, Goncalves RS, Silva CO, et al. Penetration of hydrogen peroxide and degradation rate of different bleaching products. Oper Dent 2015; 40(1): 72-79.
22. Soares DG, Basso FG, Pontes EC, Garcia Lda F, Hebling J, de Souza Costa CA. Effective tooth-bleaching protocols capable of reducing H2O2 diffusion through enamel and dentine. J Dent 2014; 42(3): 351-358.
23. Torres CR, Souza CS, Borges AB, Huhtala MF, Caneppele TM. Influence of concentration and activation on hydrogen peroxide diffusion through dental tissues in vitro. Sci World J 2013; 2013: 193241.
24. Luque-Martinez I, Reis A, Schroeder M, et al. Comparison of efficacy of tray-delivered carbamide and hydrogen peroxide for at-home bleaching: a systematic review and meta-analysis. Clin Oral Investig 2016; 20(7): 1419-1433.
25. Naik S, Tredwin CJ, Scully C. Hydrogen peroxide tooth-whitening (bleaching): review of safety in relation to possible carcinogenesis. Oral Oncol 2006; 42(7): 668-674.
26. Tredwin CJ, Naik S, Lewis NJ, Scully C. Hydrogen peroxide tooth-whitening (bleaching) products: review of adverse effects and safety issues. Br Dent J 2006; 200(7): 371-376.