Effect of Helium/Oxygen Plasma on the Shear Bond Strength of Self-adhesive Resin Cement on Coronal Dentin

Objectives: This study investigated the effects of helium/oxygen plasma on shear bond strength (SBS) when RelyX™ U200 self-adhesive resin cement was applied to coronal dentin, both when the dentin surface was re-wetted and when it was not re-wetted.

Materials and Methods: RelyX™ U200 self-adhesive resin cement was applied to coronal dentin, using the following five methods: dentin surface with no plasma treatment (control group); helium plasma jet (He); helium/oxygen plasma jet (He/O2); helium jet with re-wetting (He+R); and helium/oxygen jet with re-wetting (He/O2+R). The shear bond strength (SBS) was measured 24 hours after bonding. Water contact angles and SEM evaluations of each group, both after surface treatment and of cement/dentin interfaces, were investigated. One-way ANOVA and Sheffe Post Hoc test statistics were used.

Results: Mean SBS values in both groups treated with plasma followed by re-wetting (He+R: 16.25±2.39 and He/O2+R: 18.15±1.93 MPa) were higher than in the plasma-only (He: 13.26±1.52 and He/O2: 12.98±1.94 MPa) and control groups (11.20±1.87 MPa) (p<0.05). The results for the two plasma systems were not significantly different from each other, with or without re-wetting (p>0.05). Water contact angle measurements in all plasma-treated groups showed significantly lower contact angle values. SEM evaluations showed that plasma treatments followed by re-wetting could partially remove the smear layer and open the dentinal tubules, resulting in resin tag formation at the interfaces.

Conclusion: The use of helium/oxygen plasma jet followed by re-wetting enhances the bonding effectiveness of RelyX™ U200 self-adhesive resin cement to coronal dentin.

1. Burgess JO, Ghuman T, Cakir D, Swift Jr.E.J. Self-adhesive resin cements. J Esthet Restor Dent 2010; 22(6): 412-419.

2. Radovic I, Monticelli F, Goracci C, Vulicevic ZR, Ferrari M. Self-adhesive resin cements: a literature review. J Adhes Dent 2008; 10(4): 251-258.

3. Holderegger C, Sailer I, Schuhmacher C, Schlapfer R, Hammerle C, Fischer J. Shear bond strength of resin cements to human dentin. Dent Mater 2008; 24(7): 944-950.

4. Viotti RG, Kasaz A, Pena CE, Alexandre RS, Arrais CA, Reis AF. Microtensile bond strength of new self-adhesive luting agents and conventional multistep systems. J Prosthet Dent 2009; 102(5): 306-312.

5. Peutzfeldt A, Sahafi A, Flury S. Bonding of restorative materials to dentin with various luting agents. Oper Dent 2011; 36(3): 266-273.

6. Monticelli F, Osorio R, Mazzitelli C, Ferrari M, Toledano M. Limited decalcification/diffusion of self-adhesive cements into dentin. J Dent Res 2008; 87(10): 974-979.

7. de Munck J, Vargas M, van Landuyt K, Hikita K, Lambrechts P, van Meerbeek B. Bonding of an auto-adhesive luting material to enamel and dentin. Dent Mater 2004; 20(10): 963-971.

8. Mazzitelli C, Monticelli F, Osorio R, Casucci A, Toledano M, Ferrari M. Effect of simulated pulpal pressure on self-adhesive cements bonding to dentin. Dent Mater 2008; 24(9): 1156-1163.

9. Mazzitelli C, Monticelli F, Toledano M, Ferrari M, Osorio R. Dentin treatment effects on the bonding performance of self-adhesive resin cements. Eur J Oral Sci 2010; 118(1): 80-86.

10. Liu Y, Liu Q, Yu QS, Wang Y. Nonthermal atmospheric plasmas in dental restoration. J Dent Res 2016; 95(5): 496-505.

11. Dong X, Li H, Chen M, Wang Y, Yu Q. Plasma treatment of dentin surfaces for improving self-etching adhesive/dentin interface bonding. Clin Plasma Med 2015; 3(1): 10-16.

12. Dong X, Ritts AC, Staller C, Yu Q, Chen M, Wang Y. Evaluation of plasma treatment effects on improving adhesive-dentin bonding by using the same tooth controls and varying cross-sectional surface areas. Eur J Oral Sci 2013; 121(4): 355-362.

13. Jiang C, Schaudinn C, Jaramillo DE, Webster P, Costerton JW. In vitro antimicrobial effect of a cold plasma jet against Enterococcus faecalis biofilms. ISRN Dent 2012; 2012: 295736.

14. Koban I, Matthes R, Hübner NO, et al. Synergistic effects of nonthermal plasma and disinfecting agents against dental biofilms in vitro. New J Phys 2010; 12(7): 073039.

15. Ritts AC, Li H, Yu Q, et al. Dentin surface treatment using a non-thermal argon plasma brush for interfacial bonding improvement in composite restoration. Eur J Oral Sci 2010; 118(5): 510-516.

16. Rupf S, Lehmann A, Hannig M, Schafer B, Schubert A, Feldmann U. Killing of adherent oral microbes by a non-thermal atmospheric plasma jet. J Med Microbiol 2010; 59(Pt 2): 206-212.

17. Liu H, Chen J, Yang L, Zhou Y. Long-distance oxygen plasma sterilization: effects and mechanisms. Appl Surf Sci 2008; 254(6): 1815-1821.

18. Gauthier M, Stangel I, Ellis T, Zhu X. Oxygen inhibition in dental resins. J Dent Res 2005; 84(8): 725-729.

19. Han GJ, Kim JH, Chung SN, et al. Effects of non-thermal atmospheric pressure pulsed plasma on the adhesion and durability of resin composite to dentin. Eur J Oral Sci 2014; 122(6): 417-423.

20. Kim JH, Han GJ, Kim CK, et al. Promotion of adhesive penetration and resin bond strength to dentin using non-thermal atmospheric pressure plasma. Eur J Oral Sci 2016; 124(1): 89-95.

21. Hirata R, Teixeira H, Ayres AP, et al. Long-term adhesion study of self-etching systems to plasma-treated dentin. J Adhes Dent 2015; 17(3): 227-233.

22.Yaopromsiri C, Yu LD, Sarapirom S, Thopan P, Boonyawan D. Effect of cold atmospheric pressure He-plasma jet on DNA change and mutation. Nucl Instrum Meth Phys Res Sect B Beam Interact Mater At 2015; 365(part A): 399-403.

23. Lehmann A, Rueppell A, Schindler A, et al. Modification of enamel and dentin surfaces by non-thermal atmospheric plasma. Plasma Process Polym 2013; 10(3): 262-270.

24. von Woedtke T, Reuter S, Masur K, Weltmann KD. Plasmas for medicine. Phys Rep 2013; 530(4): 291-320.

25. Ferracane JL, Stansbury JW, Burke FJT. Self-adhesive resin cements - chemistry, properties and clinical considerations. J Oral Rehabil 2011; 38(4): 295-314.

26.Guarda GB, Gonçalves LS, Correr AB, Moraes RR, Sinhoreti MA, Correr-Sobrinho L. Luting glass ceramic restorations using a self-adhesive resin cement under different dentin conditions. J Appl Oral Sci 2010; 18(3): 244-248.

27. Chen M, Zhang Y, Sky Driver M, Caruso AN, Yu Q, Wang Y. Surface modification of several dental substrates by non-thermal, atmospheric plasma brush. Dent Mater 2013; 29(8): 871-880.

Emyoo P, Kanjantra P, Boonyawan D, Sastraruji T. Effect of Helium/Oxygen Plasma on the Shear Bond Strength of Self-adhesive Resin Cement on Coronal Dentin: Original articles. CM Dent J [Internet]. 2020 Jan 01 [cited 2024 Nov 18];42(1):101-109. Available from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=19

Emyoo, P., Kanjantra, P., Boonyawan, D. & Sastraruji, T. (2020). Effect of Helium/Oxygen Plasma on the Shear Bond Strength of Self-adhesive Resin Cement on Coronal Dentin. CM Dent J, 42(1), 101-109. Retrieved from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=19

Emyoo, P., Kanjantra Pavisuth,Boonyawan Dheerawan and Sastraruji Thanapat. 2020. "Effect of Helium/Oxygen Plasma on the Shear Bond Strength of Self-adhesive Resin Cement on Coronal Dentin." CM Dent J, 42(1), 101-109. https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=19

Emyoo, P. et al. 2020. 'Effect of Helium/Oxygen Plasma on the Shear Bond Strength of Self-adhesive Resin Cement on Coronal Dentin', CM Dent J, 42(1), 101-109. Retrieved from https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=19

Emyoo, P., Kanjantra, P., Boonyawan, D. and Sastraruji, T. "Effect of Helium/Oxygen Plasma on the Shear Bond Strength of Self-adhesive Resin Cement on Coronal Dentin", CM Dent J, vol.42, no. 1, pp. 101-109, Jan. 2020.

Emyoo, P., Kanjantra, P., Boonyawan, D., et al. "Effect of Helium/Oxygen Plasma on the Shear Bond Strength of Self-adhesive Resin Cement on Coronal Dentin." CM Dent J, vol.42, no. 1, Jan. 2020, pp. 101-109, https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=19