Effect of Desensitizing Toothpaste and Mouthwash Containing Pro-Argin and Novamin on the Microtensile Bond Strength of Dental Adhesives to Dentin
Objective: To evaluate the effect of desensitizing toothpaste and/or mouthwash, which contains Pro-Argin or Novamin on the microtensile bond strength of etch-and-rinse or self-etch adhesive to dentin.
Material and methods: Fifty extracted human molars were used in this study. A flat dentin surface was prepared by slicing horizontally parallel to the occlusal surface at 1 mm. below the DEJ. The samples were randomly divided into five main groups (n=10). Group1, samples were brushed with distilled water (negative control group). The samples in four other main groups were immersed in 6% citric acid solution for 1 minute and then treated as follows; Group2 - samples were brushed with distilled water (positive control group), Group3 and Group5 - brushed with toothpaste containing Pro-Argin and Novamin respectively, Group4 - brushed with toothpaste containing Pro-Argin as Group3 and immersed in Pro-Argin contained mouthwash for 30 sec. Toothbrushing was performed for 1 minute twice a day. All procedures were repeated for 14 days. The samples were stored in artificial saliva at 37◦C after treatment cycles. In each main group, samples were split into two subgroups for composite build-up bonded with OptibondTM FL or ClearfilTM SE Bond. After stored in distilled water at 37◦C for 24 hours, all samples were trimmed into hourglass shape with 1 mm2 bonding area approximately. The microtensile bond strength test was performed using a universal testing machine at cross-head speed of 1 mm/min. Data were analyzed by one-way ANOVA with Dunnett’s T3 test and independent sample t-test (p<0.05).
Results: The microtensile bond strengths of both dental adhesive systems to dentin treated by desensitizing toothpaste and/or mouthwash containing Pro-Argin and Novamin were significantly lower compared with control groups, with the exception of ClearfilTM SE Bond, which had no significant difference between the dentin treated by toothpaste containing Pro-Argin to the control groups. For the dentin treated by toothpaste containing Pro-Argin and Novamin, the bond strength obtained with ClearfilTM SE Bond was significantly higher compared with OptibondTM FL.
Conclusions: The use of desensitizing toothpaste and/or mouthwash containing Pro-Argin or Novamin resulted in the mineral depositions on dentin surface and showed the negative effect on bond strength of dental adhesives. ClearfilTM SE Bond exhibited higher bond strength to dentin treated by toothpaste containing Pro-Argin and Novamin compared with OptibondTM FL. This might be due to the mineral depositions that occur on dentin surface would be able to resist the phosphoric acid etchant, which has a possibility to interfere the resin adhesion. Moreover, ClearfilTM SE Bond promoted the bond strength regarding chemical interaction between the functional monomer and inorganic ions within the mineral depositions.
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